Encapsulation of methionine in ethyl cellulose by supercritical fluids technique

20/05/2023 20:34

Thuany Naiara Silva Laurintino, P. V. de Oliveira, J. L. Dias, Thaíris K.S. Laurintino, E. Rebelatto, J. V. Oliveira, A. Bolzan

August 2023


Methionine is an amino acid of great importance for lactating dairy cows, as it directly affects the quality and quantity of milk these animals produce. The main objective of this work was to investigate the encapsulation of methionine in ethyl cellulose with ethanol and acetic acid as a mixture solvent using the GAS technique. A central composite design 23 with three central points was used to evaluate the influence of methionine/polymer ratio (1:3, 1:2, and 1:1), temperature (308, 313, and 318 K), and pressure (10, 12, and 14 MPa) in terms of particle diameter, morphology of the particles, percentage of encapsulated material, encapsulation efficiency, and yield of precipitation. The release behavior of the particles precipitated was evaluated in two fluids – simulated gastric and simulated intestinal fluids. Encapsulation efficiencies as high as 99.72 % were obtained, for which the system methionine/polymer ratio showed a negative effect (95 % confidence level).